Objective: To investigate the influence of miR-363 on cell proliferation and migration of colorectal cancer.
Methods: The expression of miR-31 in colorectal cancer tissues and colorectal cancer cell lines were measured by RT-PCR. CCK-8 and cell wound scratch assay were used to detect the cell proliferation and migration of colorectal cancer cell lines transfected by miR-363 mimics and miR-363 inhibitor. The expression of CTHRC1 in colorectal cancer cell was detected by Western blotting.
Results: The expression of miR-363 in colorectal cancer cell lines and tissues were obviously up regulated (P<0.05). The cell proliferation and migration ability of colorectal cancer cell was inhibited after transfection of miR-363 mimics. Meanwhile, the expression of CTHRC1 in miR-363 mimics group was (0.31 ± 0.07), which was significantly lower than that in miR-363 inhibition group (2.46 ± 0.37).
Conclusion: miR-363 could inhibit the cell proliferation and migration ability of colorectal cancer cell and the possible mechanism is targeting on CTHRC1.Author(s): Chaxiang Li, He-Sheng Luo, Hanqing Hu, Yufeng Tian, Yaoming Zhu