Objective: To investigate the different serum expressions of CD3+, CD4+ and CD8+ in the peripheral blood among the patients with Recurrent Spontaneous Abortion (RAS) before and after immunization therapy and to evaluate the clinical effects and pregnancy outcomes.
Methods: 157 RAS patients with negative blocking antibody in our hospital were enrolled into the study and treated with immunity therapy from September 2015 to August 2017. The blocking efficiency of T lymphocytes (CD3+, CD4+ and CD8+) in peripheral blood of patients with the lymphocyte of patients’ husband (or unrelated healthy male) as antigen target were measured by double fluorescent marker flow cytometric analysis technique.
Results: After treatment, the serum blocking efficiency of CD3+, CD4+, CD8+ were higher than before treatment, P<0.05. And the serum blocking efficiency of CD3+, CD4+, CD8+ in 37 RAS patients with positive blocking antibody after treatment were higher than before treatment or 121 RAS patients with negative blocking antibody, P<0.05. Besides, there were 18 RAS patients (14.88%) who had negative blocking antibody after treatment with successful pregnancy and 12 RAS patients (33.33%) who had positive blocking antibody after treatment with successful pregnancy.
Conclusion: Active immunotherapy can improve the blocking efficiency of CD3+, CD4+ and CD8+ in peripheral blood of recurrent spontaneous abortion patients there for helping pregnancy, which would be recommended for the clinical used as an effective treatment.Author(s): Zhao Yaning, Cao Jing, Gao Weihua