ISSN: 0970-938X (Print) | 0976-1683 (Electronic)

Biomedical Research

An International Journal of Medical Sciences


Paclitaxel induces apoptosis in osteosarcoma via promoting the production of reactive oxygen species and inhibiting the expression of cyclooxygenase-2

Objective: The present study was designed to investigate the possible molecular mechanisms of apoptosis in osteosarcoma induced by paclitaxel. Study design: Cell viability was detected in human osteosarcoma cell line HOS-732 treated with paclitaxel at different concentrations. The apoptosis and reactive oxygen species (ROS) production rates were determined by flow cytometry. COX-2 expression at mRNA and protein levels in osteosarcoma tissues were measured by quantitative real time polymerase chain reaction (RT-PCR) and Western blotting. Results: Paclitaxel can obviously inhibit the proliferation of HOS-732 cells in a dose-dependent manner. The production of ROS in HOS-732 cells was remarkably increased with the increasing concentration of paclitaxel. Moreover, paclitaxel can significantly inhibit the expression of COX-2 at mRNA and protein levels in rat osteosarcoma tissue. Furthermore, the inhibitory effects of paclitaxel on COX-2 expression at mRNA and protein levels were dose-related. Conclusion: Paclitaxel treatment can induce the apoptosis and increase the production of ROS in HOS-732 cells. Moreover, paclitaxel can decrease the expression of COX-2 in a dose-dependent manner. These findings suggested that paclitaxel-induced apoptosis in osteosarcoma could be ascribed to the increased production of ROS and the reduction of COX-2 expression.

Author(s): Ming Li, Liming Yin, Lili Wu, Yunsen Zhu, Xi Wang
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