Objective: To study the protective mechanism of mangiferin on brain inflammatory lesion in spontaneously hypertensive rats.
Methods: Twenty rats with spontaneous hypertension were randomly and evenly divided into model group and mangiferin group. Ten more male rats of the same age were selected as control group. After 2 months of drug infusion, morphological changes of brain in rats with spontaneous hypertension were observed. Meanwhile, levels of TNF-α, IL- 1β and ICAM-1 in the brain of those rats were measured by ELISA. Western blot assay was performed to detect the expression of Monocyte Chemoattractant Protein (MCP-1) and Cell Chemokine Receptor (CCR2) protein.
Results: The contents of TNF-α, IL-1β and ICAM-1 in the model group increased significantly when compared with the normal control group and were markedly higher than those in the mangiferin group. The results of Western blot assay showed that the expressions of MCP-1 and CCR2 in the model group increased significantly compared with the normal control group and were markedly higher than those in the mangiferin group.
Conclusion: Mangiferin can effectively reduce levels of inflammatory factors such as TNF-α, IL-1β and ICAM-1 in brain tissues of spontaneously hypertensive rats. It has obvious anti-inflammatory effect and its mechanism may be related to the inhibition of expression of MCP/CCR2 signaling pathway.Author(s): Zhou Su, Yufang Zhan, Yanxia Song, Guiying Zhang