Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity and repeatability, and inspect the qualities of commercial Zaocys dhumnades samples.
Methods: Modern DNA fingerprint technology was used to optimize and improve the detection method of Zaocys dhumnades recorded in Pharmacopoeia of People’s Republic of China (2015), Zaocys dhumnades DNA extraction and detection reagents were research and developed, a Zaocys dhumnades DNA test kit were developed, and detection parameters of the kit were also evaluated. Eighteen samples from Beijing, Tianjin, Changchun and Jilin Cities were detected using the kit, and a comparative test of using the kit with the method recorded in the pharmacopoeia was conducted at the same time.
Results: The Zaocys dhumnades DNA detection kit was still effective after repeated freezing and thawing 5, 10 and 20 times. Fourteen of the 18 commercial Zaocys dhumnades samples were the certified products and 4 of them were counterfeits. Repeated tests demonstrated that the specificity, stability and repeatability of the kit were exactly the same. The results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing could be completed using the kid in a common laboratory.
Conclusion: The Zaocys dhumnades identification using the Zaocys dhumnades DNA detection kit is simple, convenient, accurate and effective, suitable for the rapid detection of Zaocys dhumnades DNA.Author(s): Li Zitong, Sun Jingyu, Ai Jinxia, Li Yingnuo, Li Mingcheng, Zhang Lihua, Yuan Guangxin