Objective: We aimed to examine the expression and significance of tumor necrosis factor-a-induced protein-8 like-2 (TIPE2) in peripheral blood mononuclear cells (PBMCs) in patients with acute coronary syndrome (ACS).
Methods: TIPE2 mRNA and protein levels in PBMCs from ACS patients were detected by RT-PCR, qRT-PCR and western blot. The interleukin-10 (IL-10) and interferon-γ (IFN-γ) concentrations of blood serum were detected by ELISA. We also assessed the association between TIPE2 mRNA expression and IL-10 and IFN-γ concentrations.
Results: Our research found that TIPE2 mRNA and protein expression were decreased in ACS patients compared with stable angina pectoris (SAP) and healthy person. The level of IL-10 in ACS patients was obviously lower than that in SAP individuals and normal controls, while the level of IFN-γ in ACS patients was significantly higher than that in control subjects. Furthermore, the expression level of TIPE2 mRNA was positively correlated with IL-10 concentration.
Conclusion: Our data indicate that reduced TIPE2 expression may contribute to the pathogenesis of ACS.