Constipation is a common clinical symptom and it is a highly frequent complication in patients with chronic gastrointestinal disorder and intestinal tumor. The chemicals such as DMH (Dimethylhydrazine) can also induce intestinal tumor. Loperamide-induced constipation model was established to verify that chronic constipation can increase the risk of intestinal tumor. 128 Kunming mice were divided into four groups, namely, healthy, chronic constipation, intestinal tumor induced by chronic constipation and intestinal tumor with constipation after DMH (Dimethylhydrazine) induction. Our experiments are conducted to evaluate the effects of chemical and chronic constipation induction of intestinal tumor for understanding their different mechanisms. High-throughput analysis methods (RNA-seq) are used to sequence the transcriptome of mouse intestinal tissue. GO and KEGG database are used to identify metabolism pathways and related genes. Through the comparison among the different experimental groups the Differentially Expressed Genes (DEGs) are identified, respectively. The metabolic pathways and DEGS about DMH and chronic constipation induction are very different. According to the analysis of different metabolic pathways and related genes the different tumorigenesis and modulation mechanisms of chemical and chronic constipation induction are revealed.