Naglaa K Idriss
Assiut University, Egypt
Posters & Accepted Abstracts : Biomed Res
Backgrounsd: Parkinsonâs disease is the most common chronic progressive neurodegenerative disorder after Alzheimerâs disease. The effectiveness of anti-Parkinson treatments gradually diminished by the progressive degeneration of the dopaminergic terminals. The current research investigated the effect of adipose-derived mesenchymal stem cells (AD-MSCs) versus anti-Parkinson drug in a Parkinsonism rat model. Methods: Forty adult rats divided into 4 equal groups; Group I; Control group received the vehicle. Group II; Parkinsonâs disease group, received rotenone 2mg/kg daily intraperitoneally for one month. Group III received rotenone at the same previous dose then received isolated AD-MSCs on day 14. Group IV received rotenone at the same previous dose then received carbidopa/levodopa on day 14. Behavioral tests were carried out and midbrain specimens were processed for light and electron microscopy. Genetic expression of glial fibrillary acidic protein (GFAP) and nestin mRNA were assessed by real time-PCR. Lamin-B1 and vimentin genes were detected by gel electrophoresis and plasma levels of angiopoietin-2 and dopamine were measured by ELISA. Results: Rotenone induced pronounced motor deficits, neuronal and glial alterations AD-MSCs group showed improvements in the motor function and microscopic picture. Fold change of both genes (GFAP and Nestin) were decreased significantly in ADMSC and carbidopa/levodopa group compared to parkinsonâs disease. Lamin-B1and vimentin genes were highly expressed in parkinsonâs disease. Plasma levels of angiopoietin-2 and dopamine were significantly increased after treatment (P<0.001) compared to parkinsonâs disease. Conclusions: Adipose-derived-mesenchymal stem cells reduced neuronal degeneration more efficiently than the anti-parkinson drug did in a parkinsonism rat model.