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Biomedical Research

An International Journal of Medical Sciences

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Research Article - Biomedical Research (2019) Volume 30, Issue 4

Effect of high fibre food mix on lipid profile and body weight in obese subjects

Geetha K, Geetha Yankanchi M*, Netravati Hiremath

Department of Foods and Nutrition, All India Coordinated Research Project on Home Science, University of Agricultural Sciences, GKVK, India

*Corresponding Author:
M. Yankanchi
Department of Foods and Nutrition
All India Coordinated Research Project on Home Science
University of Agricultural Sciences
GKVK, India

Accepted Date: May 24, 2019

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The study aimed to assess the efficacy of developed high fibre food mix on the management of obesity. The high fibre food mix was developed by using region specific ingredients. The dietary intervention was carried out on obese rural farm women for a period of 120 days by providing 1/3 rd daily requirement of protein and energy. Impact of food mix was evaluated by assessing somatic and biochemical parameters at pre and post dietary intervention. The developed mix contained protein (15.80 ± 0.32g), fat (2.60 ± 0.12g), energy (320 kcal), carbohydrate (60.75g) and dietary fibre (29.5 ± 0.91g) per 100 g of the mix. Significant reduction in weight (65.34 ± 10.97 kg to 63.23 ± 10.60 kg) leading to reduction in body mass index (27.84 ± 4.14 to 26.95 ± 4.06) and reduction in hip circumference (105.23 ± 9.96 cm to 104.93 ± 9.90 cm) was recorded at P≤0.05 level. Significant decrease in cholesterol, low density lipoprotein and fasting blood sugar was observed from pre to post test. The food based dietary intervention with high protein and dietary fibre showed desirable effects on body weight and biochemical parameters. Hence the inclusion of millets, legumes vegetables and oilseeds in the daily diet enhances the dietary fibre intake and thus helps in management of body weight.


Obesity, Dietary intervention, Dietary fibre, Body mass index, Lipid profile.


Obesity is one of the risk factors for morbidity and mortality including type 2 diabetes, cardiovascular, osteoarthritis, malignant and metabolic diseases. The imbalance between energy intake and expenditure is the main cause of overweight and obesity. Obesity is defined as weight > 20 per cent above the ideal weight. Body Mass Index (BMI) is an anthropometric index which is calculated by person’s weight in kilograms divided by the square of his height in meters (kg/m2) for classifying subjects as overweight and obesity. Person having BMI value 23 to 24.99 are overweight and 25 and above as obese [1]. Approximately 1.5 billion adults are overweight or obese around the world and obesity is being concerned as a global epidemic [2]. Since nineties there has been an increase in over nutrition among women in India [3]. Data from NFHS-4 indicated that around 20.70 per cent of women are over nourished [4]. In India, the increased levels of obesity are primarily associated with the transformation from rural to urban lifestyle. Modern lifestyle associated with easy access to food, lack of exercise, sedentary lifestyle, calorie dense foods and excessive television viewing contribute to the development of non-communicable diseases. Dietary fibre being one of the essential components of the healthy diet has gained the important physiological and metabolic functions. The properties of fibre make the protective role in management of overweight and obesity. Considering the importance of dietary fibre rich foods, the present study was undertaken to develop and evaluate the efficacy of high fibre food mix for management of overweight and obesity among rural farm women.

Materials and Methods

Development of millet based mix

Millet based high fibre food mix was prepared by using the ingredients viz., finger millet (Eleusine coracana), little millet (Panicum sumatrense), defatted soya (Glycine max) flour, whole green gram (Vigna radiata), fenugreek seeds (Trigonella foenum-graecum), cluster bean (Cyamopsis tetragonoloba), drumstick leaves (Moringa oleifera) and curry leaves (Murraya koinigii ). All the ingredients used for the preparation of mix were procured from local market of Bengaluru. Fresh cluster bean, drumstick leaves and curry leaves were washed thoroughly, blanched for one minute and oven dried and made into flour. Further finger millet, little millet, whole green gram and fenugreek seeds were cleaned and made into flour. Millet based mix was prepared by mixing all the flours and kept airtight for further nutrient analysis and preparation of the product for use.

Nutrient analysis

Proximate composition of developed mix was analyzed by using standard Association of Official Analytical Chemists (AOAC) protocol. Further, carbohydrate content was calculated by differential method and calorific value was obtained by multiplying the carbohydrate and protein content by four and fat by nine k cal. Dietary fibre was analyzed by AOAC method by using Fibra plus instrument and micronutrients viz., iron and calcium were analyzed by atomic absorption spectrometry [5].

Selection of subjects

Randomly 240 women involved in farm activity from seven villages of Bengaluru north taluk were screened to select the overweight and obese subjects. Height, weight, waist circumference (WC) and hip circumference (HC) were the body measurements measured for the all the subjects. BMI was derived based on height and weight. Based on BMI the subjects were classified as underweight, normal, overweight and obese. Among the screened subjects a total of 60 subjects having BMI>22.99 were selected and divided into two groups (Experimental 30 and Control 30). A written consent was obtained from all the selected subjects for their willingness to participate in the dietary intervention. Blood samples of the selected subjects were drawn by a trained technician under the medical supervision and analyzed for lipid profile and Fasting Blood Sugar (FBS) under clinical setting prior to the initiation of the study.

Dietary intervention

Dietary intervention was carried out for a period of 120 days on rural obese women involved in farm activities. For experimental group (n=30), dumpling prepared from the developed high fibre food mix was given for lunch along with curry. The quantity of high fibre food mix used for the preparation of dumpling was fixed based on their average ideal body weight. The recommended dietary allowances to meet 1/3rd of their daily requirement for energy and protein were taken care while fixing the quantity of food mix. The subjects in experimental group were monitored throughout the intervention period to ensure the consumption of provided food. Further one to one counseling on foods to be included and excluded in their diet other than the food served was given to the experimental group. Audio visual aids, method demonstrations were used for group counseling. The control group (n=30) was kept neutral without any dietary intervention and counseling. Somatic and biochemical parameters were measured in both experimental and control group at pre and post level.

The data obtained was statistically analyzed. Mean and standard deviation were calculated for each studied variable. Significance level was assessed through paired “t” test in both experimental and control group.


The nutrient composition of millet-based mix is presented in Table 1. The developed food mix contains 9.10 ± 0.07g of moisture, 15.00 ± 0.32 g of protein, 2.60 ± 0.12 g of fat, 60.75 g of carbohydrate, 320 kcal energy, 3.40 ± 0.18 g of ash, 9.11 ± 0.83 g of crude fibre and dietary fibre 29.50 ± 0.91 g per 100 g. Iron and calcium was found to be 8.33 ± 0.12 and 366 ± 0.08 mg per 100 g respectively. The developed mix contains noticeably good amount of protein, low amount of fat, energy and high amount of dietary fibre and minerals.

Nutrients Quantity
Moisture (g) 9.10 ± 0.07
Protein (g) 15.00 ± 0.32
Fat (g) 2.60 ± 0.12
Total Carbohydrates (g)* 60.75
Energy (k cal)* 320
Ash (g) 3.40 ± 0.18
Crude fibre (g) 9.11 ± 0.83
Dietary fiber (g) 29.50 ± 0.91
Iron (mg) 8.33 ± 0.12
Calcium(mg) 366 ± 0.08

Table 1: Nutrient composition of high fibre food mix (100 g).

The mean height, weight, BMI, waist circumference, hip circumference of experimental and control subject’s pre and post intervention period is presented in Table 2. The mean weight of subjects in experimental group decreased from 65.34 ± 10.97 kg to 63.23 ± 10.60 kg. The decrease in weight was found to be highly significant (p=0.01%). Similarly, significant reduction in BMI of subjects in experimental group was observed (27.84 ± 4.14 to 26.95 ± 4.06) at p=0.01 per cent. In contrary the mean weight of the subjects in control group increased from 62.30 to 63.09 kg with concurrent increase in BMI, however, the increment was non-significant. In experimental group the mean WC and HC decreased from 88.43 cm to 88.20 cm and 105.23 to 104.93 cm respectively and the reduction in HC was found to be significant (p=0.05%). In control group the WC and HC were increased from 87.06 cm to 87.63cm and 104.46 cm to 105 cm respectively, however, difference was non-significant.

Anthropometric measurements Experimental group(n=30) Control group(n=30)
Pre Post ‘t’ value Pre Post ‘t’ value
Height (cm) 153.07 ± 4.53 - - 153.03 ± 6.57 - -
Weight (kg) 65.34 ± 10.97 63.23 ± 10.60 10.56** 62.30 ± 9.13 63.09 ± 9.23 -3.34 ns
BMI 27.84 ± 4.14 26.95 ± 4.06 11.27** 26.57 ± 3.30 26.91 ± 3.29 -3.41ns
WC (cm) 88.43 ± 9.57 88.20 ± 9.50 1.36ns 87.06 ± 9.02 87.63 ± 9.42 -3.32ns
HC (cm) 105.23 ± 9.96 104.93 ± 9.90 2.52* 104.46 ± 6.70 105.00 ± 6.78 -4.0 ns
WHR 0.84 ± 0.05 0.84 ± 0.05 0.10 ns 0.88 ± 0.10 0.88 ± 0.11 -0.52ns

Table 2: Impact of food based dietary intervention on anthropometric parameters.

The detailed information on the changes in the biochemical parameters that occurred in the subjects after 120 days of dietary intervention in comparison with control subject is presented in Table 3. It is evident from the table that there is statistically significant reduction in the total cholesterol, LDL, and FBS among experimental group. However, the triglyceride and VLDL showed a decrease from initial to final which was statistically not significant. Among the control subjects some of the parameters namely total cholesterol, HDL, LDL showed decrease from initial to final which was statistically nonsignificant. Similarly, there was increase in triglyceride and VLDL which was also non-significant. There was a significant decrease in FBS level from initial too final among the experimental group which was not so among the control group.

Clinical measures Experimental group (n=30) Control group (n=30)
Pre Post ‘t’ value Pre Post ‘t’ value  
Cholesterol(mg/dl) 187.87 ± 33.90 165.63 ± 27.33 6.36** 176.30 ± 26.04 169.33 ± 36.90 1.92ns  
TGL (mg/dl) 174.83 ± 85.94 173.87 ± 65.89 0.08ns 148.26 ± 57.75 149.9 ± 57.05 -0.17 ns  
HDL (mg/dl) 42.57 ± 4.17 40.67 ± 4.23 2.01ns 42.80 ± 4.90 41.46 ± 4.26 1.87 ns  
LDL (mg/dl) 110.57 ± 25.96 94.03 ± 22.14 5.05** 105.1 ± 20.94 98.96 ± 28.43 1.90 ns  
VLDL (mg/dl) 34.97 ± 17.19 34.77 ± 13.18 0.083ns 29.65 ± 11.55 29.98 ± 15.01 -0.17 ns  
Chol/HDL 4.43 ± 0.77 4.07 ± 0.55 2.93** 4.13 ± 0.49 4.09 ± 0.80 0.35 ns  
LDL/HDL 2.64 ± 0.62 2.26 ± 0.55 3.67** 2.48 ± 0.47 2.27 ± 0.63 2.02 ns  
FBS (mg/dl) 120.50 ± 53.56 102.87 ± 46.85 4.89** 176.30 ± 26.04 169.33 ± 36.90 1.72ns  

Table 3: Impact of food based dietary intervention on biochemical parameters.


In the present study the developed food mix was higher in terms of protein and dietary fibre compared to the millet based composite food mix where the protein content ranged from 10.21 to 13.42 per cent and dietary fibre ranging from 20.60 to 23.4 per cent [6]. Reported result on composite flour mix developed from millets and legumes also had lower protein (10.8 to 11.15 g/100 g) and fat content (0.67 to 1.44 g/100 g) compared to the mix developed under the study [7]. The use of soya bean and green gram in addition to millets are the contributing factors for higher protein content. Incorporation of vegetables and fenugreek seeds in addition to whole grains increased the dietary fibre content. Several epidemiological studies showed an inverse relationship between dietary fibre intake and weight loss [8]. An inverse correlation between whole grain ingestion and change in body weight of middleaged women [9]. Fibres have the ability to displace the energy from other nutrients by adding bulk and weight to the meal.

Thus, increasing the satiety and decreasing the intake. Many studies have shown that low fat, high protein diets may increase weight loss body fat mass loss satiety and mitigate reduction in fat free mass and resting energy expenditure [10-12]. In the present study the mix with low fat, moderate protein and high dietary fibre with certain modification in their meal pattern would be the contributory factor for reduction in somatic measurements among the experimental group compared to control. It has been reported that the consumption of both soluble and insoluble dietary fibre increase satiety there by decrease the intake of quantity of food which is also evident from the present study. Decrease in energy density by 10 per cent and weight loss of 1.9 kg over 3.8 months resulted from consumption of additional fibre 14 g/day [13]. Similarly, in the present study the food contained 29.5 g of fibre with low fat per meal provided in the intervention. This might be the one of the causes for reduction in the weight of the subjects in experimental group. The finding of the changes in biochemical parameters of the present study is in line with findings of other researchers [14-17]. Further, the lowering effect of millet based dietary fibre on plasma lipid concentration and FBS was observed in low glycemic index diet of lower fat content [18].


Transition in lifestyle with more consumption of junk foods and reduced intake of fruits and vegetables coupled with sedentary lifestyle are the main causes for overweight and obesity. This is because most of the junk foods are rich in fat salt, preservatives, simple sugars and less in complex carbohydrates. The intake of fibre in such foods is far behind the recommendation. However, inclusion of millets, legumes and vegetables in the daily diet enhances the dietary fibre intake. This along with modification in lifestyle would benefit in weight reduction which would aids in prevention of secondary complications.


The research team acknowledges the Indian Council of Agricultural Research (ICAR) - Central Institute for Women in Agriculture (CIWA), Government of India and University of Agricultural Sciences, Gandhi Krishi Vigyan Kendra, Bengaluru, Government of Karnataka (GOK) for funding the research.

Ethical Committee Approval

Institutional ethical committee clearance certificate was obtained prior to the initiation of the intervention study (Proposal No. AICRP FN 2018001 and approved on 10.02.2018).

Conflict of Interest

There is no conflict of interest.