The present study was undertaken to find out the biochemical marker for diagnosis of Intes-tinal ischemia. Thirty five subjects were taken as Healthy controls, Ninety patients were di-vided into four study group’s viz-Intestinal ischemia, other ischemia’s, Jaundice and Alco-holism. Presently, the diagnostic techniques are expensive, less specific and sensitive and most of them are invasive. Hence, there is requirement of some specific, sensitive and non-invasive technique for diagnosis of Intestinal ischemia. The aim of the present study was to find out better biochemical marker for diagnosis of Intestinal ischemia. Previous all studies about Alcohol Dehydrogenase were done on animals, studies about Alcohol Dehydrogenase in Intestinal ischemia in humans are still lacking. Hence this study was undertaken to find out the use of Alcohol Dehydrogenase and γ - glutamyl transpeptidase to assess the utility in diagnosis of Intestinal ischemia. The present Study revealed highly significant increased ac-tivities of Alcohol Dehydrogenase in Intestinal ischemia, other ischemia and Alcoholism when compared with Healthy controls whereas insignificant rise in Alcoholism and Other ischemia when compared with Intestinal ischemia. γ - Glutamyl transpeptidase activities are significantly increased in Intestinal ischemia, other ischemia, Jaundice and Alcoholism when compared with Healthy controls. In conclusion it is evident from our observations, that γ - Glutamyl transpeptidase is a sensitive and specific marker for diagnosis of Intestinal ische-mia.