Valerian is the most popular plant in medicine. It has been cultivated from ancient times and established itself as a profitable culture. The underground part of valerian is the source of commonly used sedatives, especially in chronic diseases. This plant is still the subject of considerable research aimed at establishing the chemical and pharmacological basis of the activity which has been clearly shown in a number of animal and clinical studies. The purpose of the study was to standardize the raw material ?f Valeriana officinalis L. cultivated in different regions of Armenia (Aparan, Ashotavan). As a material served rhizome with roots, obtained from the Valeriana officinalis L., cultivated in different regions of Armenia (Aparan, Ashotavan) in the second half of September in 2015. The commodity analysis of the raw material (volatile oil, extract, moisture, total ash) was carried out according to the requirements of WHO. The microscopic analysis was carried out with triocular electronic “Micros” microscope (10 × 40) and Olympus Digital C-3000 Zoom camera. The qualitative analysis of essential oil was carried out by GC-MS method. The research was carried out on gas chromatograph with a mass-selective spectrometer “BrukerDaltonik” (EM-640S). Unlike the pre-mountainous zone conditions of Aparan, the latter proves that in the mountainous zone conditions of Ashotavan, the plant has a developed mechanical tissue, which is displayed with the formation of sclereids. The existence of drouses is also typical to a preparation, which is an anatomical differentiative feature for the raw material harvested from Ashotavan. In the result of the research confined the quality indices of valerian cultivated in Ashotavan and Aparan (volatile oil, the total of extracts, moisture, and total ash). The analysis showed that in volatile oil of Valerian climatized in Ashotavan and in Aparan the prevailing was bornyl acetate, the quantities of which were 55.2% and 90.7%.