Sudarshan Kriya Yoga (SKY) is a breathing technique introduced by Poojya Guruji Sri Sri Ravishankar, Founder of Ved Vignan Maha Vidya Peeth, Bangalore. This study was aimed to examine the effect of SKY practice and antioxidant enzymes activities on menopausal women. One hundred and ninety subjects of age group 45-60 years, non-smokers and nonalcoholic were selected and divided into three groups. Group 1 consisting of 40 out of 190 subjects received hormone replacement therapy (HRT). The group 2 of 40 subjects received 500mg/day of vitamin E orally for 30 consecutive days.The group 3 of 60 subjects were on daily practice of SKY for cosecutive 30 days. The remaining 50 menopausal women acted as controls. Plasma MDA, erythrocyte SOD and GSHpx concentrations were assessed in all the subjects before and after 30 days of respective treatment. Serum MDA levels were significantly decreased in all the 140 subjects; whereas SOD and GSHpx were significantly elevated in the subjects of all 3 groups after 30 days of respective treatment compared to controls. However, a high significant increase in SOD and GSHpx levels with a corresponding decrease in serum MDA were evident in the subjects who were exposed to SKY compared to the subjects who had HRT or vitamin E. Our study demonstrates that practice of SKY could be beneficial to the menopausal women by increasing in vivo levels of antioxidant.