The ethanolic leaf extract was collected from Borreria hispida using five different solvents by using shindai technique. The leaf extract contained total phenol and flavonoids with 33.21 ± 0.6 mg GAE and 18.27 ± 0.08 mg QE/g, respectively which is crucial for the potential anticancer activity. The anti-oxidant and anticancer activity was performed by DPPH assay and MTT assay, respectively. The ethanolic leaf extract showed significant amount of phytochemicals with highest (81.3 ± 0.1%) radical scavenging activity. The most potent anticancer activity of ethanolic leaf extract was observed with an IC50 value of 150 μg/ml. Cytotoxicity behaviour of ethanolic leaf extract against Hep cell line were demonstrated elaborately.