Platelet concentrates can be stored for five days at 22 0C. Our objective was to increase the storage of platelets to 7 days by decreasing the storage temperature. Lower tempera-ture may minimize chances of bacterial proliferation and also maintain platelet func-tions to optimum level. The study sample iincluded 25 blood donors of both sex in State Blood Bank, Chhatrapati Shahuji Maharaj Medical University, Lucknow. Complete his-tory of donors was taken to exclude any infection and disease. The platelet concentrates were prepared by platelet rich plasma (PRP) method. The whole blood (350 ml.) was col-lected in anticoagulant Citrate Phosphate Dextrose Adenine (CPDA) triple blood bags. Random donor platelet concentrates were evaluated on day 0, day 5 and day 7 at differ-ent temperatures of storage period. Platelet swirling was present in all the units at all the temperatures on day 7 with no evidence of bacterial contamination. Comparison of the mean values of platelet count, PF 3, lactate dehydrogenase, pH, glucose and platelet ag-gregation, showed no significant difference at 22 0C and 18 0C while PF 3, pH and glu-cose level showed a significant difference on day 7 at a temperature of 16 0C.