ISSN: 0970-938X (Print) | 0976-1683 (Electronic)

Biomedical Research

An International Journal of Medical Sciences

Abstract

Construction of Absorption Filter for colorimetry and its performance characteristics

The aim of this study was to device in house a suitable absorption filter for colorimetric analyses. An absorption filter was so constructed using 40% CuSO4.5H2O solution in 8M HCl (w/v) in glass support of 2mm internal diameter. The filter was found to have a nominal wavelength of 540±0.92nm, spectral bandwidth of 41±0.82nm and a peak transmittance of 36±0.40%. The filter offered a sensitivity of 0.81±0.03 for a single beam colorimeter at 540nm. Its photometric linearity was through an absorbance unit of 0.52±0.02 while that of the manufacturer filter was through 0.92±0.03. The significantly lower photometric linearity (P < 0.05) of the filter notwithstanding, it offered a mean value for blood haemoglobin concentration of 122±20g/l which was not significantly different from 124±19g/l obtained with manufacturer filter (P > 0.05). The filter would be suitable for absorbance measurement at 540nm wavelength particularly for blood haemoglobin concentration determination in a developing economy.


Author(s): Adeeyinwo C. E. and Adedeji A. L.

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