Vitamin D plays a major role in various physiological functions and body homeostasis. The aim of this study was to examine the relationship between serum 25-hydroxyvitamin D status with lung functions and Fractional Exhaled Nitric Oxide (FeNO). 113 (55 males and 58 females) apparently healthy participants were recruited. Participants were divided into two main groups based on their gender and vitamin D levels. Group 1 included male participants categorized according to deficient vitamin D level <30 ng/ml (n=35) and sufficient vitamin D level 30-80 ng/ml (n=20). The second group included 58 female participants with deficient vitamin D level <30 ng/ml (n=19) and sufficient vitamin D level 30-80 ng/ml (n=39). Vitamin D was measured by chemiluminescence immunoassays technique, lung Function parameters were recorded by using an electronic spirometer and Fractional Exhaled Nitric Oxide (FeNO) was measured by using Niox Mino. No association of vitamin D levels was found with reduced lung functions and FeNO levels.