Acinetobacter spp. are Gram negative coccobacilli causing various nosocomial infections. They possess different types of Beta-lactamases like SHV, TEM and others which lead to treatment failure in case of infections due to this pathogen. Studying their species distribution in various infections and drug resistance pattern is hence important. Hence our study was aimed to study the different infections caused by Acinetobacter spp, in a tertiary care hospital in Eastern India and its antibiotic susceptibility pattern.