To establish a method to produce the atherosclerosis rabbit model rapidly. A total of 18 healthy male Japanese white rabbits were randomly divided into the control group (n=8) and the experiment group (n=10). Rabbits in control group were fed with regular chow diet, while rabbits in the experiment group were fed with high fat diet and injected with bovine albumin. All animals received injure on left femoral arteries by vessel sheath. After seven weeks of injury and diet, morphological characters of pathological changes were investigated, serum lipoprotein and cholesterol levels were also profiled. Serum total cholesterol (TC), triglyceride (TG), and low density lipoprotein (LDL) levels were significantly higher in experiment group than control group (P<0.05). All rabbits in experiment group developed atherosclerotic plaques in left femoral arteries where was injured by vessel sheath indicating the successful establishment of the rabbit atherosclerosis model. The combination of high fat diet, intravenous injection of bovine albumin and arterial injury resulted in a successful establishment of atherosclerosis model in rabbit within a relatively short period of time.