Varicocele is an abnormal enlargement of vein of pampiniform plexus vein draining the testis. The upward direction of flow of blood is believed to be maintained by the presence of valves within the veins. Defective valves or compression of the veins by adjacent structures can cause dilatation of the veins of pampiniform plexus leading to formation of varicocele. The present study was carried out to see the effect of varicocele on histoarchitectural changes within the wall of vein of pampiniform plexus. The formalin-fixed tissue were reprocessed for paraffin embedding and stained with haematoxylene and eosin and Weigert stains and observed under light microscope The varicocele group showed dilatation of lumen along with thickened wall. The increased thickness of wall may be due to increased in thickness of both tunica intima and tunica media. . In many cases the tunica intima showed irregular folds along with discontinuity of the endothelium. The tunica media shows increased thickness due to increased in both collagen fibers and smooth muscle cells.