Objective: This paper aimed to analyze the importance of cerebrospinal fluid (CSF) EV71 antibody on the diagnosis of patients with severe hand, foot, and mouth disease (HFMD) accompanied with encephalitis.
Method: Data were collected from 224 children patients with severe HFMD from March 2015 to March 2017. According to the occurrence and types of encephalitis, the participants were divided into control group (no encephalitis, n=72), bacterial encephalitis group (HFMD with bacterial encephalitis, n=86), and viral encephalitis group (HFMD with viral encephalitis, n=66). Serum and CSF EV71 antibody levels were detected. The sensitivity and specificity of CSF EV71 antibody for the early prediction of HFMD accompanied with encephalitis were measured using receiver operating characteristic (ROC) curves. The relationship of serum EV71 antibody on severe HFMD accompanied with encephalitis was determined based on logistic multivariate regression analysis.
Results: The serum and CSF EV71 antibody levels of the bacterial encephalitis and viral encephalitis groups were significantly higher than those of the control group (P<0.05). The bacterial encephalitis and viral encephalitis groups showed no statistical difference in their serum and CSF EV71 antibody levels (P>0.05). ROC curves showed the relatively high sensitivity and specificity of serum and CSF EV71 antibody for the early prediction of severe HFMD accompanied with encephalitis (P<0.05). Results of logistic multivariate regression analysis showed that when encephalitis complication was used as the dependent variable, all the impact factors of serum and CSF EV71 antibody exhibited statistical significance (P<0.05).
Conclusions: Severe HFMD with encephalitis complication can cause brain tissue damage among children and induce the sharp growth of serum and CSF EV71 antibody levels. The detection of serum EV71 antibody is characteristic of simple operation and small trauma. Thus, this antibody plays an important role in the early prediction of severe HFMD with encephalitis complication.Author(s): Weiwei Dai, Jun Ma, Weiwei Feng, Tingting Lin, Lijing Zhou, Wei Qin, Xuyang Wang, Zhongqiu Lu