Purpose: To observe the influence of miRNA145 on the reproduction and transfer capacities of human osteosarcoma cell line MG-63.
Method: The Lipofectamine 2000 lipofection method is adopted to transfect miRNA145 mimic into human osteosarcoma cell line MG-63; at the same time, small RNA fragments with fluorescence are transfected as the contrast for transfection efficiency detection, so as to detect the transfection efficiency with the flow cytometer. The real-time quantitative RT-PCR detection is conducted 24 h after the transfection to detect the miRNA145 expression level in MG-63 cells. The MTT test is conducted to detect the influence of miRNA145 on the reproduction capacity of MG-63 cells. The wound healing test is adopted to detect the influence of miRNA145 on the transfer capacity of MG63 cells.
Results: The Lipofectamine 2000 lipofection method is adopted for transfection of MG-63 cells, with transfection efficiency of more than 90%. The miRNA145 expression level in MG-63 cells is significantly increased 24 h after the transfection of miRNA145 mimic (P<0.05). The transfection of miRNA145 can significantly restrain the reproduction and transfer capabilities of MG-63 cells (P<0.05).
Conclusion: miRNA145 plays a regulating role in the occurrence and development of osteosarcoma by affecting the reproduction and transfer capabilities of osteosarcoma cells.Author(s): Fei Hu, FaMing Lv, PingBo Chen, Gang Lv