To identify volatile constituents in Typhonium giganteum Engl., and clarify their effects on proliferation of breast cancer T47D cell lines. Volatile constituents in Typhonium giganteum Engl. are extracted by steam distillation, and determined by GC-MS. 24, 48 and 72 h after treatment of T47D cells with different concentrations of Typhonium giganteum Engl. volatile (DJL-V) constituents, methyl thiazolyl tetrazolium (MTT) colorimetric assay, inverted optical microscopic observation, flow cytometry (FCM) and agarose gel electrophoresis are used to detect cell proliferation and apoptosis. 15 major chemical constituents are isolated and identified from DJL-V, with a detection rate of 76.41%. DJL-V can significantly inhibit T47D cell proliferation in a time- and concentration-dependent manner within a certain concentration range, and can induce its apoptosis. T47D cell apoptosis rates are 7.51%, 13.45% and 13.79%, respectively, 24, 48 and 72 h after treatment with 100 g/L DJL-V. The degree of apoptosis is positively correlated with the time. 72 h after treatment, distribution of S phase (DNA synthesis phase) cells increases significantly. DJL-V has proliferation inhibitory and apoptosis inducing effects on T47D cells; and its anti-tumor mechanism is associated with the induction of apoptosis.