The purpose of this study was to investigate if it was possible to strengthen a tooth for autologous tooth crown transplant by using the resin impregnation method already used for industrial purposes. Eightyfour upper first premolar tooth crowns were divided into three groups (n=28 each) to be kept either wet, dry for one day, or dry for one week. In addition, within each group, the teeth were allocated to resin impregnation (RE, n=14) or no resin impregnation (NRE, n=14). A compression test was carried out on all teeth in the three groups, broken the specimens were flat-polished in cross-section with #400,1000 gritstone and observed using Confocal Laser Scanning Microscopy (CLSM). The area of bonding resin was measured using ImageJ software. The mean maximum compressive strength in the RE group was significantly higher than that in the NRE group in the all tooth condition. In CLSM observation, the deepest penetration of bonding resin into the tubules was observed in crowns that had been dried for one week. Based on the findings of this study, we conclude that impregnation with bonding resin can strengthen teeth.