Gamma Aminobutyric Acid (GABA), also known as 4-aminobutyric acid, of which the molecular formula is C4H9NO2, with a relative molecular weight of 103.2. It widely exists in the cells of microorganism, animals and plants, as a kind of neuro-inhibitory transmitter in mammals, a variety of physiological and metabolic activities are involved. It is greatly demanded by the market for the special physiological function and medicinal value with its unique molecular structure: it has the function of lowering blood pressure, treating epilepsy, enhancing memory, anti-sleeping and delaying senescence, with higher physiological activity. We used Absidia as the fermentation strain, and found out the best fermentation conditions: temperature: 31°C; time: 70 h; pH value: 4.9; inoculation age: 18 h, inoculation amount: 8%, rotation speed: 170 r/min. When the fermentation test was performed according to the conditions, the molar conversion rate of GABA can reach 32.03%, this can provide theoretical support for large-scale industrial production in the future.