Dexamethasone is a widely used glucocorticoid which has been prescribed increasingly in recent years. The effects of Dexamethasone on LH, FSH and testosterone levels were investigated in present study. In this experimental work, 40 adult male Wistar rats were assigned into four tenmembered groups. The rats in the control group received saline, while the rats in experimental group were subjected to intraperitoneal injection of dexamethasone in 0.4, 0.7, and 1 mg/kg doses daily for period of 10 days. On following day of last injection, the rats were anaesthetized and plasma was drawn from their heart, FSH, LH and testosterone levels were measured and the results were analyzed using SPSS software and Dunnett test. The hormonal test of LH, FSH and testosterone was made using Pars kit and the groups were compared. In this study, no significant change was observed in the targeted hormones up to 0.7mg/kg dose, but significant changes in the concentration of LH, FSH and testosterone were found in higher doses, i.e., 1 mg/kg dose in experimental group compared to the control group (P<0.05). The results of present study indicated that frequent injection of dexamethasone has come up with multiple changes in LH, FSH and testosterone and it has borne harmful effects on the reproductive system of male rats.