The objective of the study is to observe the anti-motion sickness effect of volatile oil extract of Pinelia ternata in rats, compare the strength of anti-motion sickness action between the Pinelia ternata volatile oil extract and the dimenhydrinate, and analyze the constituents in volatile oil of Pinelia ternata. Rats with frequency is 0.05Hz, the peak speed of 240°/s2 rotated to stimulate, evoked kaolin behavior and conditioned anorexia, motion sickness severity was judged by kaolin intake and tired of drinking behavior of saccharin water. Intragastric administration of each drug group, observe inhibition on rat kaolin behavior and the rotation stimulation before and after stimulation of 0.15% saccharin drinking water continuous 3D output in 24h. Chemical constituents in volatile oil of Pinelia ternata were analyzed by capillary gas chromatographymass spectrometry (GC-MS). Results: After administration of Pinelia ternata volatile oil, kaolin intake was significantly lower than rats in the model group, Pinelia ternata extract could promote the drinking of saccharin solution, while there was no significant difference between the dimenhydrinate group and the model group. GC-MS analysis showed that volatile oil of Pinelia ternata mainly contained 15 chemical constituents. Conclusion: Chemical constituents in volatile oil of Pinelia ternata had a significant effect against motion sickness.