To establish a fingerprint of Schisandra chinensis by HPLC. The chromatographic column was Agilent ZORBAX 300SB-C18 chromatographic column (4.6 mm × 250 mm, 5 μm). The mobile phase was methanol-water, a gradient elution was conducted and the detection wavelength was at 230 nm. The sample map was analysed by software and cluster analysis method using “Chinese medicine chromatographic fingerprint similarity evaluation system 2004A”. According to the comparative study of fingerprints, we found 10 communal peaks and identify 8 peaks. The similarity between the 20 batches of medicinal herbs was compared. It was suggested that the Similarity should be 0.950 and 0.850 for superior drugs and certified drugs. The HPLC fingerprint of Schisandra chinensis has good repeatability and strong characteristics, and it can provide scientific basis for the quality evaluation of Schisandra chinensis.