In this study, we aimed to assess levels of serum 25-hydroxyvitamin D in relation to metabolic syndrome among postmenopausal women in Gorgan. The study group included 100 postmenopausal women who were referred to the different Health Centers in Gorgan. Body mass index, waist circumference, Hip, waist to hip ratio, diastolic blood pressure, triglyceride, fasting blood glucose and 25-hydroxyvitamin D levels were significantly higher in postmenopausal women with metabolic syndrome, but HDL-cholesterol was lower. Prevalence of the metabolic syndrome was 31%. There were significant differences in 25-hydroxy vitamin D of postmenopausal women with and without vitamin D deficiency. Prevalence of the vitamin D deficiency in postmenopausal women was 30%. There were significant differences in 25-hydroxy vitamin D of postmenopausal women with and without vitamin D deficiency who had metabolic syndrome. Our results show that postmenopausal status might be a predictor of metabolic syndrome in this area. Our findings suggested that vitamin D levels have no association with metabolic syndrome. There were no significant differences in vitamin D levels in postmenopausal women with and without metabolic syndrome. Vitamin D deficiency is not associated with the metabolic syndrome.