In this present investigation the ethanolic extract of Madhuca longifolia bark (EEMLB) was screened for hepatoprotective activity against D-galactosamine (d-GalN) induced hepatiotoxicity in rats. Wistar rats were divided into five groups (n=6). Hepatotoxicity in rats was achieved by intraperitoneal injection of 400mg/kg of d-GalN for five days. Silymarin (100mg/kg) was given as reference standard. The degree of protection against liver toxicity was determined by measuring the serum biochemical parameters viz. SGPT (serum alkaline phosphatase), SGOT (serum glutamine oxaloacetate transaminase), ALP (alkaline phosphatase), total cholesterol and bilirubin (Total and Direct). The result shows that d-GalN has enhanced the levels of SGPT, SGOT, ALP, total cholesterol and bilirubin. Pretreatment with EEMLB (200mg/kg B. wt. and 400 mg/kg B. wt.) brought back the altered levels of biochemical markers to the near normal levels. Histopathological studies also confirmed the hepatoprotective activity of these extracts when compared with d-GalN treated groups. It can be concluded from the result that the extract of Madhuca longifolia bark possesses hepatoprotective activity against D-GalN induced hepatotoxicity in rats.