Providencia stuartii infections are not common. There are a few reports of declared outbreaks or spreads. In our study, nosocomial dissemination by extended-spectrum β-lactamase (ESBL) producing Providencia stuartii in a variety of clinics was investigated in a short period. Nine multidrug-resistant Providencia stuartii clinical isolates were collected between October and November in 2011. Nine isolates were obtained from six patients who had been hospitalized in a variety of clinics at a university hospital in Turkey. The clonal relationship among nine isolates was determined by pulsed-field gel electrophoresis. All isolates were ESBL positive and resistant to quinolones, and we determined amikacin resistance to three strains. There were two antibiotypes, and a second PFGE profile was not observed in different types. The outbreak was due to the dissemination of one epidemic multiresistant clone of Providencia stuartii. We considered that it had been a common dissemination from a single source and the first isolate which was collected from the first patient was epidemic clone. The respiratory tract colonization of the first patient was considered to be the possible route of transmission. The resistance to amikacin occurred during treatment with aminoglycoside. It was noteworthy.