ISSN: 0970-938X (Print) | 0976-1683 (Electronic)

Biomedical Research

An International Journal of Medical Sciences

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no dif-ference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.


Author(s): Sekaran Muniandy, Koon Chu Yaiw, Jack Bee Chook, Arokiasamy Vinscent Rayappan, Kum Thong Wong, Ikram Shah Ismail, and Rajes Qvist

Abstract | Full-Text | PDF

Share this  Facebook  Twitter  LinkedIn  Google+