Objective: This research was aimed to build ischemia-reperfusion model of rats in vivo , and observe the effects of different degrees of acidification pre-treatment of respiratory acidosis on lung ischemiareperfusion injury.
Methods: 50 Sprague-Dawley rats were randomly divided into five groups: the sham operation S group, the ischemia-reperfusion group, pretreatment L group, pretreatment M group and pretreatment H group. Rats in all groups were killed in 2 h after reperfusion and ischemia. Samples of lung tissue were selected after experiments. Wet to dry weight ratio, levels of malondialdehyde, superoxide dismutase, interleukin-8 and interleukin-10 in lung tissue were detected. Pathological changes of lung tissue were observed.
Results: Compared with the sham operation group, wet to dry weight ratio, levels of malondialdehyde, superoxide dismutase, interleukin-8 and interleukin-10 in lung tissue increased obviously. Acidification pretreatment of respiratory acidosis can inhibit interleukin-8 production after ischemia-reperfusion, and lower the levels of malondialdehyde and superoxide dismutase.
Conclusion: Acidification pretreatment of respiratory acidosis can inhibit proinflammatory cytokine production after ischemia-reperfusion injury, lower inflammatory reaction level, which may protect ischemia-reperfusion injury.