ISSN: 0970-938X (Print) | 0976-1683 (Electronic)

Biomedical Research

An International Journal of Medical Sciences

Abstract

Histamine induced apoptosis in primary-cultured glioma

The study aim was to observe the apoptosis induction effects of histamine towards the Primary Cultured Human Glioma Cells (PCHGCs), and to discuss the relationships between the histamine-induced PCHGCs apoptosis and Ca2+ influx. The fresh glioma tissues obtained from the surgery were added with 10% foetal calf serum (FBS) containing 1640 nutrient solution for the cultivation. The cells in good conditions were added into 0.01 mg/ml, 0.5 mg/ml and 0.1 mg/ml histamine media for the primary cell cultivation. The Confocal Laser Scanning Microscope (CLSM) was used to detect the intracellular free Ca2+ concentration, and the Flow Cytometry (FCM) was used to detect the cell apoptosis rate under the effects of different histamine concentrations. PCHGCs that were cultured with 0.5 mg/ml and 0.1 mg/ml histamine solutions had the significant increase of intracellular free Ca2+ concentrations, which induced the apoptosis. The apoptosis occurrence was related to the histamine amount and the cultivation period in a certain extent. Histamine induced the PCHGCs apoptosis by increasing the concentration of intracellular free Ca2+.

Author(s): Bing Li, Chang Fei, Jian Zhang, Feng Guo, Aigang Sun, Linchun Huan, Shouzhong Guo, Yuhai Liu, Mei Shao, Yuquan Jiang
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