The present study aimed at investigating the oxidative stress in patients with Gestational diabetes mellitus (GDM) by estimating Haptoglobin. Blood samples were obtained from 46 GDM pregnant women and 32 controls that were attending the out patient department of M.Y.Hospital, Indore. The lipoprotein profiles, HbA1c and Haptoglobin were measured in all the samples. Significant increase in Haptoglobin as a risk factor for the development of GDM in our study subjects. Results of the present study indicate that increased Haptoglobin levels are indicators for the oxidative stress in GDM women who had body mass index ≥26 Kg/m2 as compared to controls.