Starting from the biomechanics principle, the three-dimensional (3D) composite model of ostrich foot locomotion system was established using dissection, medical scan modelling, reverse engineering (RE) and finite element (FE) analysis technology. Simulation calculation on the model under three groups of different loads was performed. Meanwhile, plantar pressure test on the in vitro ostrich foot was conducted using plantar pressure measurement system combined with dynamic image analytic system. Then, the model validity was verified by comparing experimental results with FE simulation. In the end, stress distribution of bone, cartilage and soft tissue in static standing and dynamic impact state was calculated using FE method. Result shows: In the static standing state, Von Mises Stress of bone is the biggest. For a single phalanx, maximum Von Mises Stress and peak value of contact stress decreased gradually from the proximal to the distal. In a word, the paper contains a detailed study of the FE analysis on the ostrich foot locomotion system, provide a solid foundation for the research of high speed, heavy load and shock absorption mechanisms. The paper also provides a theoretical basis for the research of robot traveling mechanisms and vehicles traversing desert or planetary terrain.