ISSN: 0970-938X (Print) | 0976-1683 (Electronic)

Biomedical Research

An International Journal of Medical Sciences

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Abstract

Effects of DMSO (Dimethyl sulfoxide) free cryopreservation with program freezing using a magnetic field on periodontal ligament cells and dental pulp tissues.

The purpose of this study was to investigate the effects of short-term cryopreservation on the root of an incomplete human tooth with periodontal ligament cells (PDL) and dental pulp tissues. Extracted teeth were cryopreserved for three months using a programmed freezer combined with a magnetic field, known as Cells Alive System “CAS”. PDL regeneration and appropriate apexogenesis after transplanting a magnetically cryopreserved immature tooth were clinically confirmed. These findings demonstrate that teeth banking with the use of a magnetic field programmed freezer can be available for future autotransplantation as a treatment modality for replacing missing teeth. PDL regeneration and appropriate apexogenesis after transplanting a magnetically cryopreserved immature tooth were clinically confirmed. These findings demonstrate that teeth banking with the use of a magnetic field programmed freezer can be available for future autotransplantation as a treatment modality for replacing missing teeth. Results indicate that short-term cryopreservation with the use of a CAS freezer does not affect the rate of recovery or characteristics of PDL cells.


Author(s): T. Kawata, S. Abedini, M. Kaku, H. Koseki, S. Kojima, H. Sumi, M. Motokawa, T. Fujita, J. Ohtani,N. Ohwada, K. Tanne

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