The purpose of this study was to investigate the effect of different warm-up protocols (Post Activation Potentiation model warm-up (PAP) and Static Stretching model warm-up) on Running-based Anaerobic Sprint Test (RAST) in male athletes. 44 male athletes age: 22.23 ± 1.85 y; height: 176.16 (5.84) cm; weight: 77.49 (11.21) kg volunteered to participate in this investigation. The athletes who participated in this study were randomly divided into three groups: Post Activation Potentiation Group (PAPG) (n=15), Static Stretching Group (SG) (n=14) and Control Group (CG) (n=15). Initial measurement values of repetitive sprint test for all athletes were made followed by 5 min passive rest after 5 min warm-up at 8 km/h on treadmill. The second measurements of the athletes were performed 48 h after the first measurements. In analyzing the data, a two-way repeated measure Analysis of Variance (ANOVA) was conducted. The results indicated that significant group χ time interactions were observed for RAST test scores in the athletes subjected to different warm-up protocols. As a result, post activation potentiation warm-up method leads to an acute increase in peak power and mean power scores after the repeated sprint test (p<0.05) while static warm-up method causes a significant decrease (p<0.05).