In this study, the effects of exogenic application of Ganoderma lucidum polysaccharide on oxidative stress and hyperlipidemia was determined in diabetics rats. The study included 60 Wistar albino rats aged 4-5 months. Rats were divided into 6 equal groups. The groups were control, Ganoderma lucidum polysaccharide, diabetic, diabetic+60 mg/kg polysaccharides, diabetic+120 mg/kg polysaccharides and diabetic+10 mg/kg polysaccharide. Diabetes was induced by intraperitoneal injection of streptozocine at the dose rate of 50 mg/kg. The rats were fed ad-libitum throughout the experiment. At the end of the study, we took some samples out of total oxidant and total antioxidant levels and measured them with the spectrophotometer; thereafter we used the auto-analyser to measure the levels of total cholesterol, triglycerides, HDL, LDL, VLDL cholesterol and pancreatic amylase. We examined the blood of diabetic fasting rats and the effect was that their glucose level was increased significantly. (p<0.05), nonetheless the dose of 60, 120 and 180 mg/kg of Ganoderma lucidum polysaccharides did not reduce the blood glucose level (p>0.05). With the diabetic rats, the dose of 60, 120 and 180 mg/kg of Ganoderma lucidum polysaccharides rides effected in the increase of serum levels of total cholesterol, triglycerides, VLDL, and HDL cholesterol levels, although it was not a significant change (p>0.05), instead, we observed a statistically significant decrease of LDL cholesterol level (p<0.05). According to this study we concluded that polysaccharides effects in the decrease of hyperlipidaemia with regard to diabetes. During the study we applied Ganoderma lucidum polysaccharides exogenously (60, 120, 180 mg/kg) and it did not affect the level of total antioxidant in diabetes significantly (p>0.05), however with a dose of 180 mg/kg the total oxidant level in pancreas and liver tissues was reduced significantly and has been considered as crucial fact. The collected data supported our view on histopathologic examination. Based on all the information, we used one type of fungus Ganoderma lucidum polysaccharides which did not influence the blood glucose level in diabetes, whereas with regard to LDL and total oxidants it played a significant role in reducing its levels.