The high-density lipoprotein-cholesterol (HDL) is well known as the “good cholesterol”, since it removes harmful “bad cholesterol” out of blood. Low HDL levels increase the risk for cardiovascular diseases. This paper presents a solution for detection of low HDL level by using artificial neural network (ANN). The big dataset with 1517 persons is used for ANN training, validation and testing. Our solution achieves positive predictive value PPV=0.6782 and negative predictive value NPV=0.7066, which means that it is suitable both for positive and negative low HDL level detection.