Rheumatoid arthritis is a chronic inflammatory disease condition in synovial joints causing progressive destruction of synovial fluid resulting moderate to severe pain. Oxidative stress plays a major role in inflammation. Free radicals which are found to have in our body, either as a product of metabolism or incorporated from our environment, plays a major role in formation of oxidative stress, that leads to inflammation. To reduce this oxidative stress anti-oxidant may contribute in management of inflammation. In this study, the antioxidant and anti-inflammatory property of tartaric acid and β- carotene has established and compared with vitamin C and diclofenac sodium as standard drug. The free radical scavenging assay and protein denaturation assay was conducted for this purpose. The % inhibition confirmed the activity of individual molecules. According to the study outcome, Tartaric Acid is more effective antioxidant and anti-inflammatory agent than β-carotene. And so far it can be used as anti-rheumatoid arthritic agent. The IC50 values and graphical representations confirm the result.