The aim of this work is to study the phytochemical and antibacterial screening of Olea sp. leaves collected from Albaha region in Saudi Arabia. Elements such as Tannins, Steroids, flavonoids, coumarines, terpenoids, anthocyanates and proteins were detected when examining the Olea sp. Plant. The antibacterial activity was carried out by agar well diffusion method; Ampicillin was used as positive control and DMSO as negative control. The minimum inhibitory concentrations (MICs) were determined by using Micro dilution method. The results concluded that the aqueous extract of the plant showed lower antibacterial activities while the Ethanolic extract of Olea sp. was the most active extract against the pathogenic bacteria. The Ethanolic extract of Olea sp. was active against Staphylococcus aureus, Streptococcus pyogenes, E. coli, Pseudomonas aeruginosa and Salmonella sp. with MIC values ranged from 31.2-62.5 μl/ml. The obtained data from the Olea sp. plant confirmed its wide application for therapeutic purposes in alternative therapy.