Background: Now-a-days, the increase in drug resistance among Acinetobacter is a major concern all over the world. The aim of this study was to investigate the antimicrobial and anti-biofilm activity of ethanolic extract and ethyl acetate of Leaf, Fruit and Stem Capparis spinose extract against Acinetobacter baumannii. Methods: Twelve strains of A. baumannii were isolated from patients who referred to hospitals in Zabol. Antibiotic resistance pattern was determined by Kirby-Bauer method. Minimum inhibitory concentration and minimum bactericide concentration were determined by microdilution method. Results: The results of the antibiotic resistance pattern showed that 100% of the samples were susceptible to CZ (Cefazolin)-GM (Gentamycin) - AZM (Azithromycin) antibiotics and only 16.6% of the samples were resistant to AM (Ampicillin) antibiotics. The results of this study showed that the lowest inhibitory concentration of the C. spinose ethanolic extract against A. baumannii was 25 ppm in which two bacterial strains were inhibited while the highest inhibitory concentration was 50 ppm and the highest concentration of fecundity was equal to 100 ppm. Conclusion: Results of this study show the antimicrobial effects of good C. spinose biofilm on A. baumannii, which can be used as an effective treatment.