The aromatic fruit, its extract and the oil of Juniperus Communis Lynn (JCL), a tree species that grows in the Mediterranean and Isparta region, have been commonly used at least for three centuries as herbal treatment and in the field of medicine. The aim of this study was to apply JCL in various doses to rats in the treatment of hypercholesterolemy and analyse the biochemical parameters and the hystopathologic effects on kidney tissue. In this study 35 adult male Wistar albino rats of 200-250 gr in weight were used. The rats were divided into 5 equal groups of 7. The control group was fed with normal pellet chow, the cholesterol (Chol) group was fed with pellet chow containing 2% of cholesterol, and the 50 JCL, 100 JCL and 200 JCL groups were fed with 50, 100, 200 mg/kg JCL oil, respectively in addition to the 2% cholesterol-containing pellet chow. JCL was dissolved in 0.5% Sodium Carboxy Methyl Cellulose (SCMC) and administered by a gavage needle. The experiment was ended 30 days later and blood and kidney tissue samples were taken. A complete blood test and biochemical measurements were made. Kidney tissues were analysed histopathologically. The administration of 200 mg/kg JCL led to a significant increase in BUN and Crea levels. The administration of chol increased the TC level significantly, but the administration of JCL together with chol prevented this increase. The administration of chol led a significant increase in Ox-LDL levels. However, the administration of 200 mg/kg JCL together with chol did not result in a significant increase. At the end of the study it was found that the JCL oil showed an anti-hypercholesterolemic effect. While no anemic effect and no distinct change overall were observed in all groups, a distinct focal damage was observed in the tubular cast structure.