The present paper describes a comparative characterization of cytotoxic effects of doxycycline and cisplatin on osteosarcoma cells by using impedance sensing techniques. The impedance sensing device was obtained from Applied Physics and the osteosarcoma cells were cultured on the electrodes of the purchased devices. The impedimetric cytotoxic natures of both the drugs on cells are investigated by using impedance analyzer at 10 mV in a frequency range of 100 Hz to 1 MHz. Impedance spectrum decreases drastically in drug treated samples above 10 μM dose as cell death and detachment from the electrode surfaces increases rapidly. However the cisplatin shows better results over doxycycline as observed from impedimetric as well as conventional results. The cisplatin treated sample shows lesser impedance than the doxycycline treated sample as the IC50 value of doxycycline is more than cisplatin on osteosarcoma cells.