The purpose of this study is to investigate acute effects of 800 meters middle distance running on oxygen saturation and some metabolic parameters in adult females. Twenty female volunteers preparing for sports academy were included in the study. The mean age of the participants was 19.72 y, mean height was 160.00 cm and mean body weight was 52.45 kg. All the participants completed a middle distance running exercise 2 h a day and 4 days in a week over the period of 8 months. Choice Med Finger pulse oximeter was utilized in order to measure capillary oxygen saturation of the participants before and after the running test. Inbody-720 bioelectrical impedance analyzer was used for the measurement of metabolic parameters. Paired Sample T-test was conducted for the comparison of the participants’ mean measurements before and after running. The values of SO2 pre-test: 98.09 ± 1.01 post-test: 96.36 ± 1.17, Mineral pre-test: 2.93 ± 0.23, post-test: 2.90 ± 0.23, bone mineral content pre-test: 2.43 ± 0.19, post-test: 2.40 ± 0.20 were found to be statistically significant (p<0.01). When the acute effects of 800 m middle distance running on capillary oxygen saturation and metabolic parameters in adult females are considered, it can be stated that there have been serious decreases in values of SO2, mineral and bone mineral content compared to those measured before running. It can be suggested that in order for female middle distance runners to protect themselves from low bone mineral density, mineral-rich diets and mineral supplements may be useful.