ISSN: 0970-938X (Print) | 0976-1683 (Electronic)

Biomedical Research

An International Journal of Medical Sciences

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

A simple RP-HPLC method for determining imidacloprid residues in goat tissues.

The present work is aimed to develop a rapid, simple, sensitive and reliable reverse phase high performance liquid chromatography (RP-HPLC) method of imidacloprid and its residues in different substrates of goat tissues. Separation was achieved using C-18 column with mobile phase consisting of acetonitrile and purified HPLC grade water. The method consists of extracting with acetonitrile from different tissues and cleans up with acetonitrile and hexane (50%) mixture. Quantification was performed by reverse phase (RP) HPLC with PDA detector. The chromatogram showed a well-resolved peak of imidacloprid, and the retention time was 3.30 minutes. The limit of detection and limit of quantification were 0.01 μg/mland 0.04 μg/ml respectively and recovery from different substrates was 92-98%. Therefore, the method is satisfactory and proved to be very precise.


Author(s): Gayatri Dewangan, Akhilesh Mishra, Tapan Kumar Mandal

Abstract | Full-Text | PDF

Share this  Facebook  Twitter  LinkedIn  Google+