The present work is aimed to develop a rapid, simple, sensitive and reliable reverse phase high performance liquid chromatography (RP-HPLC) method of imidacloprid and its residues in different substrates of goat tissues. Separation was achieved using C-18 column with mobile phase consisting of acetonitrile and purified HPLC grade water. The method consists of extracting with acetonitrile from different tissues and cleans up with acetonitrile and hexane (50%) mixture. Quantification was performed by reverse phase (RP) HPLC with PDA detector. The chromatogram showed a well-resolved peak of imidacloprid, and the retention time was 3.30 minutes. The limit of detection and limit of quantification were 0.01 μg/mland 0.04 μg/ml respectively and recovery from different substrates was 92-98%. Therefore, the method is satisfactory and proved to be very precise.